anti akr1c3 mouse monoclonal antibody  (R&D Systems)

Journal: Drug Design, Development and Therapy

Article Title: Rhein Alleviates Cisplatin-Induced Acute Kidney Injury via Downregulation of NOX4-COX2/PGFS Signaling Pathway

doi: 10.2147/DDDT.S515409

Figure Lengend Snippet: Molecular docking of Rhein with NOX4, COX2 and PGFS as well as CETSA and database validation. ( A–C ) A structural view of the interaction of Rhein with NOX4, COX2 and PGFS. Rhein is shown as a yellow stick representation. Details on the binding site interactions are shown in the down panel. Non-polar hydrogen atoms are hidden for clarity. Potential intermolecular hydrogen bonds are shown as yellow dashed lines. ( D ) Rhein treatment (40 μM) increases the thermal stability of NOX4, COX2 and PGFS in cell lysates as measured by the temperature-dependent cellular thermal shift assay. ( E ) External validation datasets were obtained from transcriptome sequencing data of renal cortical in CDDP-induced AKI or CDDP-treated HK2 cells, including GEO datasets GSE85957 , GSE37133 , GSE147625 , GSE153625 and GSE98602 . Data are shown as mean ± SEM. Data are shown as mean ± SEM. *P < 0.05, *** P < 0.001, **** P < 0.0001: CDDP vs Control.

Article Snippet: Antibodies against Bcl2 (68,103-1-Ig), cleaved-caspase-3 (68,773-1-Ig), p65 (80,979-1-RR), p-p65 (82,335-1-RR), NOX4 (14,347-1-AP), PGFS (AKR1C3, 11,194-1-AP) and β-actin (81115-1-RR) were purchased from Proteintech Group (Wuhan, China).

Techniques: Biomarker Discovery, Binding Assay, Thermal Shift Assay, Sequencing, Control

Journal: Drug Design, Development and Therapy

Article Title: Rhein Alleviates Cisplatin-Induced Acute Kidney Injury via Downregulation of NOX4-COX2/PGFS Signaling Pathway

doi: 10.2147/DDDT.S515409

Figure Lengend Snippet: Molecular dynamic (MD) simulations of Rhien with NOX4, COX2 and PGFS. ( A ) Root-mean-square deviation (RMSD) curve of legend (Rhein, green line), protein (NOX4, blue line), and the NOX4-Rhein complex (red line). ( B ) Root-mean-square fluctuation (RMSF) curve of NOX4. ( C ) Radius of gyration (Rg) curve of NOX4. ( D ) Analysis of solvent accessible surface area (SASA) curve of NOX4. ( E ) RMSD curve of legend (Rhein, green line), protein (COX2, blue line), and the COX2-Rhein complex (red line). ( F ) RMSF curve of COX2. ( G ) Rg curve of COX2. ( H ) SASA curve of COX2. ( I ) RMSD curve of legend (Rhein, green line), protein (PGFS, blue line), and the PGFS-Rhein complex (red line). ( J ) RMSF curve of PGFS. ( K ) Rg curve of PGFS. ( L ) SASA curve of PGFS.

Article Snippet: Antibodies against Bcl2 (68,103-1-Ig), cleaved-caspase-3 (68,773-1-Ig), p65 (80,979-1-RR), p-p65 (82,335-1-RR), NOX4 (14,347-1-AP), PGFS (AKR1C3, 11,194-1-AP) and β-actin (81115-1-RR) were purchased from Proteintech Group (Wuhan, China).

Techniques: Solvent

Journal: Drug Design, Development and Therapy

Article Title: Rhein Alleviates Cisplatin-Induced Acute Kidney Injury via Downregulation of NOX4-COX2/PGFS Signaling Pathway

doi: 10.2147/DDDT.S515409

Figure Lengend Snippet: Rhein reduces CDDP-induced AKI by targeting inhibition of NOX4, COX2 and PGFS. A single dose of CDDP (20 mg/kg, i.p.) was administered to the C57BL/6 mice to induce acute kidney injury, and Rhein (80 mg/kg/day) was administered by oral gavage for three consecutive days in mice after intraperitoneal injection with CDDP. ( A ) NOX4, COX2 and PGFS in renal cortex were measured by Western blot and corresponding quantification analysis (n = 5). ( B ) NF-κB activation-related proteins p-p65/p65 and p-IκBα/ IκBα in renal cortex were measured by Western blot and corresponding quantification analysis (n = 5). ( C ) Apoptosis -related proteins p-p65/p65, Bcl2, BAX and cleaved-caspase-3 in renal cortex were measured by Western blot and corresponding quantification analysis (n = 5). Data are shown as mean ± SEM. **P < 0.01, ***P < 0.001: CDDP vs Control. # P < 0.05, ## P < 0.01: CDDP+Rhein vs CDDP.

Article Snippet: Antibodies against Bcl2 (68,103-1-Ig), cleaved-caspase-3 (68,773-1-Ig), p65 (80,979-1-RR), p-p65 (82,335-1-RR), NOX4 (14,347-1-AP), PGFS (AKR1C3, 11,194-1-AP) and β-actin (81115-1-RR) were purchased from Proteintech Group (Wuhan, China).

Techniques: Inhibition, Injection, Western Blot, Activation Assay, Control

Journal: Drug Design, Development and Therapy

Article Title: Rhein Alleviates Cisplatin-Induced Acute Kidney Injury via Downregulation of NOX4-COX2/PGFS Signaling Pathway

doi: 10.2147/DDDT.S515409

Figure Lengend Snippet: Rhein mitigated CDDP-induced HK2 cell damage at least in part by downregulation of NOX4-NF-kB-COX2/PGFS signaling pathway. CDDP (10μg/mL)-treated HK2 cells were incubated with 40 μM Rhein for 24 h. ( A ) NOX4, COX2 and PGFS in HK2 cells were measured by Western blot and corresponding quantification analysis (n = 6). ( B ) Anti-apoptotic protein Bcl2, and pro-apoptotic proteins BAX and cleaved-caspase-3 in HK2 cells were measured by Western blot and corresponding quantification analysis (n = 6). ( C ) NF-κB activation-related proteins p-p65/p65 and p-IkBα/ IkBα in HK2 cells were measured by Western blot and corresponding quantification analysis (n = 6). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001: CDDP vs Control. # P < 0.05, ## P < 0.01: CDDP+Rhein vs CDDP.

Article Snippet: Antibodies against Bcl2 (68,103-1-Ig), cleaved-caspase-3 (68,773-1-Ig), p65 (80,979-1-RR), p-p65 (82,335-1-RR), NOX4 (14,347-1-AP), PGFS (AKR1C3, 11,194-1-AP) and β-actin (81115-1-RR) were purchased from Proteintech Group (Wuhan, China).

Techniques: Incubation, Western Blot, Activation Assay, Control